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Identification of Candida Species Screened from Catheter Using Patients with PCR-RFLP Method

ABSTRACT
Candiduria or presence of infecting Candida species in urinary tract is one of the usual hospital infections. Factors
increasing susceptibility of patients to such infections are including high utilization of antibiotics, long
hospitalization periods, higher ages, diabetes mellitus, sexuality, female sex and use of immunosuppressive therapy.
This study focuses not only on diagnosis of candiduria and its causes using phenotype and genotype analyzing
methods, but also discusses on frequencies of Candiduria in catheter using patients. 250 samples of catheter using
patients were collected and cultured in biological media. Identifying the yeasts, initially some phenotypic
experiments were carried out such as germ tube, the production of hyphae or pseudohyphae and clamydospores in
CMA+TW80 medium, and CHROMagar Candida. Genomic DNA of all species were extracted and analyzed with
PCR and subsequent Polymerase Chain Reaction – Restriction Fragments Length Polymorphism (PCR-RFLP)
methods. Analyzing urination of 95 female and 155 male, 40 samples were recognized as infected that demonstrates
۵۵% and 45% Candiduria infection in females and males. Frequency of C. albicans, C. glabrata, C. tropicalis, C.
parapsilosis, and C. krusei are 45%, 32.5%, 15%, 5%, and 2.5%, respectively. Candiduria frequency is measured to
be about 16%

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Etiologic Agents of Candidemia in Pediatric Immunocompromised.pdf

Abstract Background: Candidemia is the main cause of fungal nosocomial bloodstream infections and is related …

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