Fusarium solani, as the most predominant agent of mycokeratitis, may also
occur as a mixed infection with HSV. Furthermore due to similarities in their clinical
features, sometimes it leads to misdiagnosis. The aim of this research ,was the rapid
and accurate detection of F. solani as a main etiological agent or as a mixed infection in
cases suspected to HSV. Accordingly, 65 negative, and 35 positive samples for HSV were
collected. LAMP technique with (TEF-1α) as the target gene , was developed for detection
of F. solani. The test was carried out in 1-h reaction at 65 °C in a heater block . Using this
method for the total, 2 cases among the negative samples were found to be positive for F.
solani. All were rechecked by PCR and the results were the same. The specificity of the
test was 100% and its sensitivity was one copy of genome. It showed that, both methods
were equal for this purpose. However, due to advantages of the LAMP technique, it can be
a substitute for PCR, even in low technology laboratories. Besides this can be concluded,
some cases suspected to herpeskeratitis ,are related to F. solani, which could be detected
by molecular methods.